Hippex is an excellent centrifugation extender.
The use of Hippex semen extender for fresh or chilled semen renders centrifugation of diluted semen to remove the plasma in most cases unnecessary. Even without centrifugation, the storage capacity of the extender is beyond expectation, while the fertility of the diluted sperm cells is at least at an acceptable level.
However, when the concentration of sperm cells in the ejaculate is too low, the semen can be diluted with an equal volume of Hippex extender and then be subjected to centrifugation. Thereafter, the concentrated sperm cells can be diluted with Hippex extender to the desired concentration for fresh use or cooled transport.
The procedure for freezing equine sperm cells usually begins with diluting the freshly obtained ejaculate with an equal volume of centrifugation extender. The diluted semen is then centrifuged and the thus obtained pellet with sperm cells is taken up in a so-called freezing extender.
Hippex was found by several expert users in different countries to be best commercially available extender for the dilution and subsequent centrifugation of semen, in order to obtain the best freezing results.