Hippex Centrifugation Medium (HCM).
The use of Hippex semen extender for fresh or chilled semen
renders centrifugation of diluted semen to remove the plasma in most cases unnecessary. Even without centrifugation, the storage capacity of the extender is beyond expectation, while the fertility of the diluted sperm cells is at least at an acceptable level.
However, when the concentration of sperm cells in the ejaculate is too low, the semen can be diluted with an equal volume of HCM and then be subjected to centrifugation. Thereafter, the concentrated sperm cells can be diluted with Hippex extender or with HCM to the desired concentration for fresh use or cooled transport.
The procedure for freezing equine sperm cells usually begins with diluting the freshly obtained ejaculate with an equal volume of HIPPEX Centrifugation Medium. The diluted semen is then centrifuged and the thus obtained pellet with sperm cells is taken up in HIPPEX CRYO extender.
HIPPEX, HIPPEX CENTRIFUGATION MEDIUM and HIPPEX CRYO were found by several expert users in different countries to be best commercially available extenders for the dilution and subsequent centrifugation of semen, in order to obtain the best freezing results.